TASK1 and TASK3 in orexin neuron of lateral hypothalamus contribute to respiratory chemoreflex by projecting to nucleus tractus solitarius

TWIK-related acid-sensitive potassium channels (TASKs)-like current was recorded in orexin neurons the lateral hypothalamus (LH), which are essential respiratory chemoreflex. However, specific mechanism responsible for pH-sensitivity remains elusive. Thus, we hypothesized that TASKs contribute to In present study, found TASK1 and TASK3 were expressed neurons. Blocking or microinjecting acid artificial cerebrospinal fluid (ACSF) LH stimulated breathing. contrast, alkaline ACSF inhibited breathing, attenuated by blocking TASK1. Damage of stimulatory effect on respiration caused microinjection (at a pH 6.5) antagonists. The orexinA-positive fiber type 1 receptor (OX1R) located nucleus tractus solitarius (NTS). exciting acidosis OX1R NTS. Taken together, conclude sense extracellular change through regulate projecting